Qualitative Detection of Triterpenes and Quantification of Betulinic Acid from Hexane Extract of Simarouba glauca Leaves by Gas Chromatography-Mass Spectrometry and High-Performance Thin Layer Chromatography
نویسندگان
چکیده
The present study focuses on the efficiency of high-performance thin layer chromatography and gas chromatography-mass spectrometry analysis in qualitative quantitative triterpenoid from terpene fraction hexane extract Simarouba glauca leaves. total terpenoid content was assessed which shows that 73.69 % is terpenoid. extracted petroleum ether leaves subjected to analysis. Gas results indicated seventeen compounds out only one triterpene (Squalene an important bioactive triterpenoid). In separation achieved by using hexane:ethyl acetate a 5:5 proportion as mobile phase anisaldehyde sulphuric acid reagent used for derivatization imparted violet color containing bands. Detection quantification betulinic other triterpenes done densitometric scanning at 525 nm. Betulinic produced compact spots Rf 0.66. Linear range prepared concentrations 1-7 μg/spot with correlation coefficient R2 standard deviation 0.96004 %±19.68 respectively. presence 10 triterpenoids showing different values including acid. This confirmed
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ژورنال
عنوان ژورنال: Indian Journal of Pharmaceutical Sciences
سال: 2022
ISSN: ['0250-474X', '1998-3743']
DOI: https://doi.org/10.36468/pharmaceutical-sciences.1036